International Database for Barley Genes and Barley Genetic Stocks
BGS 275, Leafless 1, lfl1
Stock number: BGS 275
Locus name: Leafless 1
Locus symbol: lfl1
Previous nomenclature and gene symbolization:
Leafless = lfl (3).
Leafless 1.a = lfs1.a (1).
Leafless 2.b = lfs2.b (1).
Monofactorial recessive (2, 3).
Located in chromosomes 6HL (1, 2); lfl1.b is associated with SNP markers 1_0297 to 1_0128 (positions 85.71 to 180.85 cM) in 2H bins 07 to 11, with SNP markers 1_0676 to 1_1534 (positions 44.96 to 180.69 cM) in 6H bins 03 to 11, and with SNP markers 2_0247 to 2_1223 (positions 159.73 to 198.70 cM) in 7H bins 09 to 12 of the Bowman backcross-derived line BW477 (1); the F2-derived line for lfl1.a (BW476) contains many markers from GSHO 609, but donor parents SNP markers were retained in both BW lines only in the long arm of chromosome 6H (1, 2).
The leafless 1 mutants are characterized by a reduction in the size of leaf blades. Leaf blades on lower culm phytomers are normal while those associated with upper phytomers of the culm are gradually shortened until the blade of the flag leaf is commonly less than 2 to 3 cm in length. Other morphological traits appear unaffected by the lfl1 mutant (2, 4). The original leafless mutant (GSHO 609) showed a much stronger reduction in leaf blade development with only a few partially developed leaf blades on lower culm leaf sheathes. The nearly complete loss of leaf blades was associated with numerous tillers of which only a few elongating and producing spikes (2). The second leafless mutant lfl1.b could be an allele based on phenotypic in the BW lines, but allelism tests have not been conducted (2).
Origin of mutant:
An induced mutant (KM 255, GSHO 609) in Kanto Bansei Gall [this cultivar likely is Golden Melon (OUJ818, CIho 958)] based on notes from T. Tsuchiya (3).
lfl1.a in Golden Melon (OUJ818, CIho 958) from Okayama University, Kurashiki, Japan (3); lfl1.b in an unidentified breeding line, which likely originated from KM 255, from the Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan (4).
Mutant used for description and seed stocks:
lfl1.a (KM 255, GSHO 609) in Golden Melon (OUJ818, CIho 958); lfl1.b in an unidentified breeding line; lfl1.a from GSHO 609 in Bowman (PI 483237)*1 (BW 476, NGB 22155); lfl1.b from an unidentified breeding line in Bowman*2 (BW 477, NGB 22156).
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Hang, A. 1995. List of genetic stocks in barley arranged in numerical order of BGS (Barley Genetic Stock) numbers. USDA-ARS National Small Grains Germplasm Research Facility, Aberdeen, ID 83210. (Notes from T. Tsuchiya on the collection of barley genetic stocks at Colorado State University.)
4. Takeda, K. (Personal communications).
J.D. Franckowiak 2017. Barley Genet. Newsl. 47:111-112.