International Database for Barley Genes and Barley Genetic Stocks
BGS 86, Chlorina seedling 13, fch13
Stock number: BGS 86
Locus name: Chlorina seedling 13
Locus symbol: fch13
Previous nomenclature and gene symbolization:
Chlorina seedling 13 = f13 (3).
Monofactorial recessive (3).
Located in chromosomes 5HL (1, 2); the plant tested from the Bowman backcross-derived line BW355 was heterozygous for fch13.v gene and retained heterozygous SNP markers only from 1_0600 to 2_0402 (positions 269.6 to 297.9 cM) in 5H bins 13 to 15 (1, 2). Although two small regions in 2HL and 5HL of BW355 were homozygous for SNP markers (1), collection of a leaf sample from a plant homozygous for fch13.v would have been very difficult (2).
The mutant is classified as white seedlings (devoid of chlorophyll and carotenoid pigments) and a heterozygous stock is maintained in the USDA-ARS Barley Genetic Stocks Collection. Mutant plants die at the seedling stage if planted in the field. When grown in the greenhouse, however, seedlings gradually develop a pale green color, leaves have a light green color at heading, and plants survive to maturity and produce plump seed (3). The Bowman backcross-derived line for fch13.v, BW355, failed to head in field trials (2), but maintenance as a homozygous line is possible when grown under greenhouse conditions (2).
Origin of mutant:
A spontaneous mutant in Nigrinudum (NSL 3286, GSHO 988) (3).
fch13.v (Nigrinudum II, GSHO 16) in Nigrinudum (NSL 3286, GSHO 988) (3).
Mutant used for description and seed stocks:
fch13.v (GSHO 16) in Nigrinudum; fch13.v in Bowman (PI 483237)*6 (BW355, NGB 20594).
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Hang, A. 1993. Trisomic analysis on the chlorina mutant B2-4. Barley Genet. Newsl.22:23-24.
A. Hang and J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:124.
J.D. Franckowiak. 2014. Barley Genet. Newsl. 44:77.