International Database for Barley Genes and Barley Genetic Stocks
BGS 623, Eligulum-a, eli-a
Stock number: BGS 623
Locus name: Eligulum-a
Locus symbol: eli-a
Previous nomenclature and gene symbolization:
Eligulum-a = lig-a (3.
Eligulum-3 = eli-3 (5).
Eligulum-3 = eli-3 (5).
Inheritance:
Monofactorial recessive (3, 6).
Located in chromosome 2HS (6); eli-a.17 mapped in 2HS 2.2 cM proximal of SNP marker 2_0964 at (position 17.85 cM) (6); eli-a.18 mapped in 2HS 1.6 cM proximal of SNP 3_1284 (19.47 cM) (6); Bowman backcross-derived lines for eli-a.3 (BW295), eli-a.9 (BW296), and eli-a.216 (BW293) retained donor parent markers in this region of 2HS (1).
Located in chromosome 2HS (6); eli-a.17 mapped in 2HS 2.2 cM proximal of SNP marker 2_0964 at (position 17.85 cM) (6); eli-a.18 mapped in 2HS 1.6 cM proximal of SNP 3_1284 (19.47 cM) (6); Bowman backcross-derived lines for eli-a.3 (BW295), eli-a.9 (BW296), and eli-a.216 (BW293) retained donor parent markers in this region of 2HS (1).
Description:
Plants do not have ligules in the junction between the sheath and leaf blade, auricles are rudimentary and asymmetrically displaced. Plants are about 2/3 of normal height and have very wide leaves (4, 5). The peduncle is short and spike emergence from the sheath of the flag leaf is poor. Spikes have a compact arrangement of spikelets and are extremely compacted near the tip (2, 4). The culm breaks very easily between the upper and lower halves of the nodes. The Bowman backcross-derived lines have glume awns that are nearly twice as long as those of Bowman, but the lemma awns are about 2/3 of normal (2). The eli-a.17 and eli-a.18 mutants increased tillering of uniculm 2 (cul2) plants (6). ELI-A produce shorter plants with fewer tillers and disrupt the leaf blade-sheath boundary, producing liguleless leaves and reduced secondary cell wall development in stems and leaves. Presence of ELI-A transcripts at the preligule boundary, suggesting that Eli-a contributes to boundary formation between the blade and sheath (6).
 with typical eligulum formation'))
 with typical eligulum formation'))
 rudimentary and asymmetrically displaced auricle'))
 rudimentary and asymmetrically displaced auricle with missing ligula'))
 culm with rudimentary and asymmetrically displaced auricle and missing ligula'))
 mutant leaf sheath to the left compared with normal barley'))
 allele to the left compared with two cultivar Steptoe culms. Pictures take in Andy Kleinhofs department, Pullman, USA.'))
Origin of mutant:
An ethylene imine induced mutant in Foma (NGB 14659, CIho 11333) (3, 4).
Mutational events:
eli-a.2 (NGB 115389), eli-a.3 (NGB 115390), -a.7 (NGB 115392), -a.9 (NGB 115393), -a.10 (NGB 115394) in Foma (NGB 14659, CIho 11333) (5); eli-a.11 (NGB 115395), -a.14 (NGB 115397) in Kristina (NGB 14661, NGB 1500); eli-a.15 (NGB 115398), -a.16 (NGB 151399) in Bonus (NGB 14657, PI 189763) (5), eli-a.216 (FN216, GSHO 3647) in Steptoe (CIho 15229) (2, 4). ); eli-a.17 and eli-a.18 are sodium azide induced mutants in a Bowman cul2.b-rob1.a backcross-derived line (BW206, NGB 22034)
Mutant used for description and seed stocks:
eli-a.3 (NGB 115390) in Foma; eli-a.3 in Bowman (PI 483237, NGB 22812)*4 (BW295, NGB 20579); eli-a.9 from Foma in Bowman*2 (BW296, NGB 20580); eli-a.14 from Kristina in Bowman*3 (BW294, NGB 20578); eli-a.216 from Steptoe in Bowman*3 (BW293, NGB20577).
References:
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Gustafsson, Å., A. Hagberg, U. Lundqvist, and G. Persson. 1969. A proposed system of symbols for the collection of barley mutants at Svalöv. Hereditas 62:409-414.
4. Kleinhofs, A. (Unpublished).
5. Lundqvist, U. (Unpublished).
6. Okagaki, R.J., A. Haaning, H. Bilgic, S. Heinen, A. Druka, M. Bayer, R. Waugh, and G.J. Muehlbauer. 2018. ELIGULUM-A regulates lateral branch and leaf development in barley. Plant Physiol. 176:2750-2760.
2. Franckowiak, J.D. (Unpublished).
3. Gustafsson, Å., A. Hagberg, U. Lundqvist, and G. Persson. 1969. A proposed system of symbols for the collection of barley mutants at Svalöv. Hereditas 62:409-414.
4. Kleinhofs, A. (Unpublished).
5. Lundqvist, U. (Unpublished).
6. Okagaki, R.J., A. Haaning, H. Bilgic, S. Heinen, A. Druka, M. Bayer, R. Waugh, and G.J. Muehlbauer. 2018. ELIGULUM-A regulates lateral branch and leaf development in barley. Plant Physiol. 176:2750-2760.
Prepared:
U. Lundqvist and J.D. Franckowiak. 2002. Barley Genet. Newsl. 32:126.
Revised:
J.D. Franckowiak and A. Kleinhofs. 2005. Barley Genet. Newsl. 35:192.
U. Lundqvist and J.D. Franckowiak. 2007. Barley Genet. Newsl. 37:290.
U. Lundqvist and J.D. Franckowiak. 2013. Barley Genet. Newsl. 43:164.
U. Lundqvist and J.D. Franckowiak. 2014. Barley Genet. Newsl. 44:199.
J.D. Franckowiak and U. Lundqvist. 2018. Barley Genet. Newsl. 48:170-171.
U. Lundqvist and J.D. Franckowiak. 2007. Barley Genet. Newsl. 37:290.
U. Lundqvist and J.D. Franckowiak. 2013. Barley Genet. Newsl. 43:164.
U. Lundqvist and J.D. Franckowiak. 2014. Barley Genet. Newsl. 44:199.
J.D. Franckowiak and U. Lundqvist. 2018. Barley Genet. Newsl. 48:170-171.
