International Database for Barley Genes and Barley Genetic Stocks
BGS 674, Mottled leaf 8, mtt8
Stock number: BGS 674
Locus name: Mottled leaf 8
Locus symbol: mtt8
Previous nomenclature and gene symbolization:
Mutant 1661 (2).
Monofactorial recessive (2, 3).
Location is unknown (3). The Bowman line for mtt8.1661 (BW598) differs from Bowman only for SNP markers near the Rph3 (reaction to Puccinia hordei 3) locus (1).
The mtt8.1661 seedlings display chlorotic bands, which are more pronounced on the first leaf. Under long day conditions, the bands do not appear on the later emerging leaves and the plants eventually seem to recover normal phenotype. Under short day conditions, the mutation is semi-lethal since all leaves develop the characteristic chlorotic bands and the plants fail to reach maturity and produce seeds. However, the phenotype was initially more severe under long day conditions (2, 3). The cell walls of leaf blade tissues that display the bleached phenotype in mtt8.1661 plants are highly autofluorescent and the fluorescence is more pronounced in the vascular tissues. The green tissues of the same mutant show little or no autofluorescence. The cell walls are clearly thickened in lesioned tissue (3). The mtt8.1661 mutant showed enhanced resistance to the P. hordei isolate that was virulent on Rph3.c, with no pustules on bleached tissues and very few on surrounding green tissue of the first leaf (3). The mtt8.1661 plants were more susceptible to Pyrenophora teres f. teres than Bowman Rph3.c based on counting the number of spores produced on leaf surfaces (3).
Origin of mutant:
A fast neutron induced mutant in Bowman Rph3.c (Bowman*11//Estate/3.2 uz als MM) (BW746, PI 643152, NGB 22452) (2, 3).
mtt8.1661 (NGB 23523, GSHO 3597) in Bowman Rph3.c (Bowman*11//Estate/3.2 uz als MM) (PI 643152, NGB 22452) (2, 3).
Mutant used for description and seed stocks:
mtt8.1661 (NGB 23523, GSHO 3597) in Bowman Rph3.c (BW746, PI 643152, NGB 22452); mtt8.1661 from Bowman Rph3.c in Bowman (PI 483237) (BW598, NGB 22164).
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2010. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Wright, S.A.I., M. Azarang, and A.B. Falk. 2007. Four new barley mutants. Barley Genet. Newsl. 37:34-36.
3. Wright, S.A.I., M. Azarang, and A.B. Falk. 2013. Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch. Plant Path. 62:982-992.
S.A.I. Wright, A.B. Falk, J.D. Franckowiak, and U. Lundqvist. 2013. Barley Genet. Newsl. 43:182.