International Database for Barley Genes and Barley Genetic Stocks
BGS 461, Zebra stripe 2, zeb2
Stock number: BGS 461
Locus name: Zebra stripe 2
Locus symbol: zeb2
Previous nomenclature and gene symbolization:
Zoned leaf = zd (4).
Zoned leaf = z (5, 7, 14).
Zoned leaf c = zbc (7, 13, 15).
Zebra leaf or zoned Colorado = zc (6, 14).
Zebra striped leaves = zd (8).
Zebra stripe 2 =zb2 (14).
Chlorina seedling 10 = f10 (3, 11, 15).
Monofactorial recessive (4, 5, 11).
Located in chromosome 4HS (3, 4); zeb2.b is between the lgn4 (light green 4) and lgn3 (light green 3) loci (9); zeb2.b is about 9.3 cM distal from the Kap1 (Hooded lemma 1) locus (3, 10); zeb2.b is about 17.0 cM proximal from the glf3 (glossy leaf 3) locus (3, 10); no recombination with the lgn4 locus (3, 10); zeb2.b is associated with SNP markers 1_1180 to 1_0946 (positions 58.13 to 74.11 cM) in 4H bin 05 of the Bowman backcross-derived line BW931 (1); fch10.s is associated with SNP markers 1_0668 to 1_1224 (positions 63.19 to 91.93 cM) in 4H bins 05 and 06 of the Bowman backcross-derived line BW352 (1), in 4H bin 05 near the centromere.
Leaves are chlorina throughout the life cycle, independent of light and temperature (4). Plants may be killed by low temperature during the winter when normal plants are unaffected (4). A zonate pattern of different degrees of chlorina is observed in seedling leaves. After much confusion about this gene, the locus was named zebra stripe 2 = zb2 (14). Plants are very weak or non-viable in the field in North Dakota, but only reduced vigor is noted when grown in the greenhouse (2). When compared to Bowman in the field, the Bowman backcross-derived line for zeb2.b (BW931) headed about 11 days later and produced only little very thin seed. Rachis internodes were slightly shorter and plants were 3/5 to 3/4 of normal height (2).
Origin of mutant:
Spontaneous mutants in an unknown cultivar (4, 15).
zeb2.b (GSHO 93) in an unknown cultivar (4, 5, 12, 14); fch10.s in an unknown six-rowed cultivar (GSHO 1234) (10, 11); based on SNP patterns the fch10.s mutant studied by Woodward (10, 15) originated in six-rowed barley and the zeb2.b mutant first isolated by Immer and Henderson (4) originated in Bonus or a closely related line (1).
Mutant used for description and seed stocks:
zeb2.b (GSHO 93) in an unknown two-rowed cultivar; zeb2.b in Bowman (PI 483237)*4 (GSHO 2012); zeb2.b in Bowman*7 (BW931, NGB 22360); fch10.s (GSHO 1737) in an unknown six-rowed cultivar; fch10.s in Bowmnan (PI 483237)*7 (BW352, NGB 20591).
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Hayashi, J., T. Konishi, I. Moriya, and R. Takahashi. 1984. Inheritance and linkage studies in barley. VI. Ten mutant genes located on chromosomes 1 to 7, except 3. Ber. Ohara Inst. landw. Biol., Okayama Univ. 18:227-250.
4. Immer, F.R., and M.T. Henderson. 1943. Linkage studies in barley. Genetics 28:419-440.
5. Kasha, K.J., and G.W. Walker. 1960. Several recent barley mutants and their linkages. Can. J. Genet. Cytol. 2:397-415.
6. Robertson, D.W. 1964. New genes in barley with their relation to linkage groups and chromosomes. p. 159-180. In S. Broekhuizen, G. Dantuma, H. Lamberts, and W. Lange (eds.) Barley Genetics I. Proc. First Int. Barley Genet. Symp., Wageningen, 1963. Centre for Agricultural Publications and Documentation, Wageningen.
7. Robertson, D.W., G.A. Wiebe, and R.G. Shands. 1947. A summary of linkage studies in barley: Supplement I, 1940-1946. J. Am. Soc. Agron. 39:464-473.
8. Robertson, D.W., G.A. Wiebe, R.G. Shands, and A. Hagberg. 1965. A summary of linkage studies in cultivated barley, Hordeum species: Supplement III, 1954-1963. Crop Sci. 5:33-43.
9. Smith, L. 1951. Cytology and genetics of barley. Bot. Rev. 17:1-55, 133-202, 285-355.
10. Takahashi, R., J. Hayashi, T. Konishi, and I. Moriya. 1975. Linkage analysis of barley mutants. Barley Genet. Newsl. 5:56-60.
11. Takahashi, R., J. Hayashi, and I. Moriya. 1973. Two new mutant genes on chromosome 4. Barley Genet. Newsl. 3:65-66.
12. Tsuchiya, T. 1975. Allelic relationships of some morphological and chlorophyll mutants in barley. Barley Genet. Newsl. 5:64-67.
13. Tsuchiya, T. 1983. Proposed new gene symbol for two different mutant types for zebra Colorado, or zoned leaf. Barley Genet. Newsl. 13:84.
14. Tsuchiya, T. 1984. Zebra striped leaves: Problems in gene and stock designation and proposed new symbols for zoned or zebra mutants in barley. Barley Genet. Newsl. 14:21-24.
15. Woodward, R.W. 1957. Linkages in barley. Agron. J. 49:28-32.
T.E. Haus. 1984. Barley Genet. Newsl. 14:93 as BGS 177, Chlorina 10, f10.
T. Tsuchiya. 1984. Barley Genet. Newsl. 14:98 as BGS 461, Zebra or zoned leaves 2, zb2.
J.D. Franckowiak and A. Hang. 1997. Barley Genet. Newsl. 26:407-408.
J.D. Franckowiak. 2013. Barley Genet. Newsl. 43:152-153.