International Database for Barley Genes and Barley Genetic Stocks

BGS 608, Proanthocyanidin-free 28, ant28

BGN  46:131
Stock number: BGS 608
Locus name: Proanthocyanidin-free 28
Locus symbol: ant28

Previous nomenclature and gene symbolization:



Monofactorial recessive (5).
Located in chromosome 3HL, ant28.484 is close to the Est4 (Esterase 4) locus (1). ant28.494 mapped about 11 cM proximal from microsatellite marker EBmag0705 in the distal region of 3HL (2).


Mutant plants synthesize a wild type amount of anthocyanins in their vegetative parts. The testa layers of the mutant grains lack catechins and proanthocyanidins. The ant28 gene affects the proanthocyanidin specific branch of the flavonoid pathway, but the exact nature of the gene action is not known (4). The gene for the R2R3 MYB domain protein, which regulates proanthocyanidin accumulation in developing grains, was isolated by PCR from the barley mutant ant28.494 and named Hvmyb10 due to its similarity to Tamyb10 or R-1 of wheat (2). The ant28 mutants evaluated had reduced grain dormancy indicating that Hvmyb10 is a key factor in grain dormancy in barley (2).
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Origin of mutant:

A sodium azide induced mutant in Grit (NGB 13685, PI 548764) (3).

Mutational events:

ant28.484 (NGB 13711) in Grit (NGB 13685, PI 548764(5); ant28.493 (NGB 21978), 28.494, 28.495 (NGB 21979) in Catrin (5); ant28.2092 (NGB 21980), 28.2093 (NGB 21981), 28.2095 (NGB 22827) in Nairn (5); ant28.2131 (NGB 13712), 28.2132 (NGB 21982) in Alexis (NGB 13688, PI 564487) (5).

Mutant used for description and seed stocks:

ant28.484 (NGB 13711) in Grit; ant28.2131 (NGB 13712) in Alexis.


1. Garvin, D.F., J.E. Miller-Garvin, E.A. Viccars, J.V. Jacobsen, and A.H.D. Brown. 1998. Identification of molecular markers linked to ant28-484, a mutation that eliminates proanthocyanidin production in barley seeds. Crop Sci. 38:1250-1255.

2. Himi, E., Y. Yamashita, N. Haruyama, T. Yanagisawa, M. Maekawa, and S. Taketa. 2012. Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley. Euphytica 188:141-151.

3. Jende-Strid, B. 1978. Mutation frequencies obtained after sodium azide treatments in different barley varieties. Barley Genet. Newsl. 8:55-57.

4.Jende-Strid, B. 1993. Genetic control of flavonoid biosynthesis in barley. Hereditas 119:187-204.

5. Jende-Strid, B. 1993. Coordinator's report: Anthocyanin genes. Barley Genet. Newsl. 22:136-137.


B. Jende-Strid. 1999. Barley Genet. Newsl. 29:99.


J. Franckowiak and U. Lundqvist. 2016. Barley Genet. Newsl. 46:131.