International Database for Barley Genes and Barley Genetic Stocks
BGS 656, Brachytic 13, brh13
Stock number: BGS 656
Locus name: Brachytic 13
Locus symbol: brh13
Previous nomenclature and gene symbolization:
Brachytic-p = brh.p (5).
Brachytic-ac = brh.ac (2, 5).
Monofactorial recessive (5, 7).
Located in chromosome 5HS (1, 2, 3); brh13.p is approximately 8.7 cM distal from SSR marker Bmag0387 in 5H bin 03 (1). brh13.p is associated with SNP markers 2_1324 to 2_1121 (positions 47.40 to 105.91 cM) in 5H bins 02 to 06 of the Bowman backcross-derived line BW084 (3); the Bowman backcross-derived line for brh13.ac (brh18.ac), BW089, was not evaluated by Druka et al. (3); the brh13 (HvCPD) locus is positioned at 44.24 cM (2) on the barley genome map (9, 10).
Plants of the brh134.p mutant are about 2/3 normal height and thewir awns are about 1/2 normal length. Peduncles and leaf blades are about 2/3 and 3/4 normal length, respectively (1, 6). The length of the rachis internodes is about 5/6 that of Bowman, 5.8 vs 4.3 mm. The spikelets at the tip of the spike are close together giving a fascinated appearance. Seedling leaves of brh13.p plants are relatively short. Plants lodge relatively easily (1, 6). Kernels of the Bowman backcross-derived line for brh13.p, BW084, were about the same size as those of Bowman, but kernel weights averaged about 20% less and test weight was lower. BW084 had erect growth habit and their grain yields averages about 1/2 of those Bowman (6). The brh13.p mutant shows a brassinosteroid deficient phenotype including reduced culm length due to short upper internodes, irregular rachis internode length, short awns, acute leaf angles, and strongly undulating leaf margins (2). The brh13.p mutant is at the brh13 (Brachytic 13) or CONSTITUTIVE PHOTOMORPHOGENIC DWARF (HvCPD) locus, which encodes the barley C-23α-hydroxylase cytochrome P450 90A1 (CYP90A1) (2).
Origin of mutant:
A sodium azide induced mutant in Birgitta (NGB 14667, NGB 1494, NSGC 1870) (8).
brh13.p (18:02:4, DWS1013, GSHO 1681) in Birgitta (NGB 14667, (NGB 1494, NSGC 1870) (6, 7). brh13.ac (brh18.ac) (402B, DWS1277, GSHO 1670) in Mo6/4*Triumph (CIho 11612, GSHO 2465) (2, 4, 5, 7); based on allelism tests conducted by Dockter et al. (2) brh18.ac (BGS659) is renamed brh13.ac.
Mutant used for description and seed stocks:
brh13.p (GSHO 1681) in Birgitta; brh13.p in Bowman (PI 483237)*6 (GSHO 2174, BW084, NGB 20491). brh13.ac (GSHO 1670) in Mo6/4*Triumph; brh13.ac in Bowman*6 (GSHO 2182, BW089, NGB 22474).
1. Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005. Characterization and molecular mapping of genes determining semidwarfism in barley. J. Hered. 96:654-662.
2. Dockter, C., D. Gruszka, I, Braumann, A. Druka, I. Druka, J. Franckowiak, S.P. Gough, A. Janeczko, M. Kurowska, J. Lundqvist, U. Lundqvist, M. Marzec, I. Matyszczak, A.H. Müller, J. Oklestkova, B. Schulz, S, Zakhrabekova, and M. Hanson. 2014. Induced variations in brassinosteroid genes define barley height and sturdiness, and expand the green revolution genetic toolkit. Plant Physiol. 166:1912-1927.
3. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendrarnin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
4. Falk, D. 1985. (Personal communications).
5. Franckowiak, J.D. 1995. The brachytic class of semidwarf mutants in barley. Barley Genet. Newsl. 24:56-59.
6. Franckowiak, J.D. (Unpublished).
7. Franckowiak, J.D., and A. Pecio. 1992. Coordinator’s report: Semidwarf genes. A listing of genetic stocks. Barley Genet. Newsl. 21:116-127.
8. Lehmann, L.C. 1985. (Personal communications).
9. Mayer, K.F. , M. Martis, P.E. Hedley, H. Simková, H. Liu, J.A. Morris, B. Steuernagel, S. Taudien, S. Roessner, H. Gundlach, M. Kubalákoyá, P. Suchánková, F. Murat, M. Felder, T. Nussbaumer, A. Graner, J. Salse, T. Endo, H. Sakai, T. Tanaka, T. Itoh, K. Sato, M. Platzer, T. Matsumato, U. Schotz, J. Dolezel, R. Waugh, and N. Stein. 2011. Unlocking the barley genome by chromosomal and comparative genomics. Plant Cell 23:1249-1263.
10. The International Barley Genome Sequencing Consortium. 2012. A physical, genetic and functional sequence assembly of the barley genome. Nature 491:711-716.
J.D. Franckowiak and L.S. Dahleen. 2007. Barley Genet. Newsl. 37:296.
J.D. Franckowiak. 2015. Barley Gewnet. Newsl. 45:234-235.