International Database for Barley Genes and Barley Genetic Stocks

BGS 676, Chlorina-a, clo-a

BGN  48:173
Stock number: BGS 676
Locus name: Chlorina-a
Locus symbol: clo-a

Previous nomenclature and gene symbolization:

Chlorina-117 = clo-117 (3, 5).
Chlorina-117 = clo.117 (1).
Chlorina seedling 16 = fch16 (4).


Monofactorial recessive (3, 5).
Located in chromosome 2HS (1); clo-a.117 is associated with SNP markers 2_1187 to 2_1338 (positions 51.52 to 74.97 cM) in 2H bin 05 to 06 and also with a few SNP markers 2_0119 to 1_0510 (positions 144.81 to 149.26) in 4HL bin 10 of the Bowman backcross-derived line BW185 (1).


Seedling and immature leaves of the clo-a.117 mutant are pale yellow-green (chlorophyll deficient) in color and gradually become darker. A slight yellow green color may be observed prior to heading (2, 3). Leaf blades of the Bowman backcross-derived line for clo-a.117 (BW185) were slightly wider than Bowman leaf blades. The spikes of BW185 plants had observed to have slightly few kernels than those of Bowman in some trials. The BW185 plants were 10 to 20% shorter than Bowman and showed about a four day delay in anthesis. Kernel weights and grain yields were about normal (2). The locus name clo-a and the allele symbol clo-a.117 are suggested for this Bonus mutant because no other named chlorina seedling mutant mapped in chromosome 2HL has similar phenotypic characteristics (1, 2).

Origin of mutant:

A sodium azide induced mutant in Bonus (NGB 14657, PI 189763) isolated by U. Lundqvist (3, 6).

Mutational events:

clo-a.117 (NGB 22623) in Bonus (NGB 14657, PI 189763) (3, 4, 5). The chlorina (clo) mutant stocks isolated in Scandinavia are stored in the Biology Department, Lund University with Mats Hansson who is the coordinator for chlorophyll biosynthesis (5, 6).

Mutant used for description and seed stocks:

clo-a.117 (NGB 22623) in Bonus; clo-a.117 in Bowman (PI 483237, NGB 22812)*5 (BW185, NGB 22017).fch16.117 (NGB 22623) in Bonus; fch16.117 in Bowman (PI 483237, NGB 22812)*5 (BW185, NGB 22017).


1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2010. Genetic dissection of barley morphology and development. Plant Physiol.155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Lundqvist, U. (Unpublished).
4. Lundqvist, U., and J.D. Franckowiak. 2018. Chlorina seeding 16, revised. Barley Genet. Newsl. 48:173.
5. Simpson, D.J., O. Machold, G. Høyer-Hansen, and D. von Wettstein. 1985. Chlorina mutants of barley (Hordeum vulgare L.). Carlsberg Res. Commun. 50:223-238.
6. Simpson, D.J., and D. von Wettstein. 1992. Coordinator’s report: Nuclear genes affecting the chloroplast. Stock list of mutants kept at Carlsberg Laboratory. Barley Genet. Newsl. 21:102-108.


J.D. Franckowiak and U. Lundqvist. 2010. Barley Genet. Newsl. 40:144.


U. Lundqvist and J.D. Franckowiak. 2018. Barley Genet. Newsl. 48:173.
U. Lundqvist and J.D. Franckowiak. 2019. Barley Genet. Newsl. 49: