BGS 14, Desynapsis 5, des5

Hordeum vulgare Disrupted Meiotic cDNA 1 = HvDMC1 (1).

Monofactorial recessive (5, 6.).
Located in chromosome 5H (1, 2); des5.e is associated with SNP markers 2_0139 to 2_1363 (positions 194.97 to 198.70 cM) in 7H bin12 and with SNP markers 1_1198 to 2_1275 (positions 73.70 to 104.73) in 5H bins 04 to 06 of the Bowman backcross-derived line BW243 (2); in the centromeric region of chromosome 5H (1).

The chromosomes are paired during pachytene and undergo desynapsis during diplotene. The degree of desynapsis is 11.1 ± 2.6 ranging from 7 ring bivalents (d = 0) to 14 univalents (d = 14). Many univalents split longitudinally during anaphase I. Lagging chromosomes and micronuclei are observed frequently at telophase I. Microspore quartets contain an average of 4.5 micronuclei per quartet with a range of 0 to 18. Ovule fertility is about 7%. When crossed with non-allelic desynaptic lines, the F1's frequently show a low degree of desynapsis (up to 3 rod bivalents per cell) (4). Under field conditions, plants of the Bowman backcross-derived line for des5.e, BW243, exhibited variable seed set with grain yields from 1/4 to 1/2 those of Bowman. Plant heights of BW243 ranged from 90% of Bowman to the same height and kernels weights ranged from 80% of those for Bowman to almost the same (3). HvDMC1 RNA was observed in all tissues, but was higher in young 5 mm developing inflorescence and highest in 1 to 1.5 cm inflorescences corresponding to pre-meiosis and meiosis stages, respectively (1).

A spontaneous mutant in Betzes (PI 129430) (5, 6).

des5.e (GSHO 596), des5.f, des5.g in Betzes (PI 129430) (5, 6).

des5.e (GSHO 596) in Betzes; des5.e in Bowman (PI 483237, NGB22812)*4 (BW243, NGB 22470).

1. Colas, I., A. Barakate, M. Macaulay, M. Schreiber, Jr Stephens, S. Vivera, C. Halpin, R. Waugh, and L. Ramsay. 2019. desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley. J. Exp. Bot. 70:2683-2698.
2. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
3. Franckowiak, J.D. (Unpublished).
4. Hernandez-Soriano, J.M. 1973. Desynaptic mutants in Betzes barley. M.S. Thesis. Univ. of Arizona, Tucson.
5. Ramage, R.T., and J.M. Hernandez-Soriano. 1971. Desynaptic genes in Betzes barley. Barley Genet. Newsl. 1:38.
6. Ramage, R.T., and J.M. Hernandez-Soriano. 1972. Desynaptic genes in barley. Barley Genet. Newsl. 2:65-68.

J.M. Hernandez-Soriano, R.T. Ramage, and R.F. Eslick. 1973. Barley Genet. Newsl. 3:128.

J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:59.
J.D. Franckowiak. 2011. Barley Genet. Newsl. 41:73.
J.D. Franckowiak. 2014. Barley Genet. Newsl. 44:56.
J.D. Franckowiak. 2014. Barley Genet. Newsl. 48:59-60