International Database for Barley Genes and Barley Genetic Stocks
BGS 222, Necrotic leaf spot 1, nec1
Stock number: BGS 222
Locus name: Necrotic leaf spot 1
Locus symbol: nec1
Previous nomenclature and gene symbolization:
Mutant no. 10 (4).
Parkland spot = sp,,b (2).
Parkland spot = sp,,b (2).
Inheritance:
Monofactorial recessive (4, 6).
Located in chromosome 1HL (2, 4, 6); nec1.c is near the centromere (2); nec1.c is about 34.5 cM proximal from the wst5 (white streak 5) locus (5, 8) and about 10.0 cM distal from the msg1 (male sterile genetic 1) locus (6, 9); nec1.c is near EST marker BF630384 in 1H bin 09 (10); nec1.c is associated with SNP markers 1_0516 to 2_0657 (positions 96.74 to 115.07 cM) in 1H bins 09 to 10 of the Bowman backcross-derived line BW628 (1); nec1.i is associated with SNP markers 2_1072 to 2_0625 (positions 55.85 to 157.02 cM) in 1H bins 06 to 12 of the Bowman backcross-derived line BW627 (1), in 1H bin 09 (10).
Located in chromosome 1HL (2, 4, 6); nec1.c is near the centromere (2); nec1.c is about 34.5 cM proximal from the wst5 (white streak 5) locus (5, 8) and about 10.0 cM distal from the msg1 (male sterile genetic 1) locus (6, 9); nec1.c is near EST marker BF630384 in 1H bin 09 (10); nec1.c is associated with SNP markers 1_0516 to 2_0657 (positions 96.74 to 115.07 cM) in 1H bins 09 to 10 of the Bowman backcross-derived line BW628 (1); nec1.i is associated with SNP markers 2_1072 to 2_0625 (positions 55.85 to 157.02 cM) in 1H bins 06 to 12 of the Bowman backcross-derived line BW627 (1), in 1H bin 09 (10).
Description:
Small black-brown spots develop on all light-exposed parts of the plant starting near the leaf tip at the three-leaf stage (2, 4). The spots are oval (the longest dimension is parallel to the leaf veins) and generally less than 1 to 2 mm in size. The spots are concentrated in awn and the most distal parts of the leaf blade, but may occur on all plant parts (4, 6). No other consistent agronomic or morphological differences between BW628 (nec1.c) and Bowman were observed (3). The nec1 locus is an orthologue of Arabidopsis necrotic mutant HLM1 that encodes the cyclic nucleotide-gated ion channel 4 and over-expresses the pathogenesis-related gene PR-1 (10). Keiša et al. (7) suggest that the nec1.c mutation alters activation of systemic acquired resistance-related physiological markers and non-host resistance while not altering compatible interaction with host pathogens.
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Origin of mutant:
A mutant induced by combined treatment with gamma-rays and diethyl sulfate of Carlsberg II (NGB 5085, CIho 10114) (4).
Mutational events:
nec1.a (Mutant no 10, GSHO 989) in Carlsberg II (NGB 5085, CIho 10114) (4, 5); nec1.c (sp,,b, GSHO 1284) in Parkland (CIho 10001) (2, 6); a mutant in Morex (CIho 15773) (8); nec1.i (FN338, GSHO 3602) in Morex (CIho 15773) (10); nec1.j (FN085, GSHO 3603) and nec1.y (FN370, GSHO 3604) in Steptoe (CIho 15229) (10).
Mutant used for description and seed stocks:
nec1.a (GSHO 989) in Carlsberg II; nec1.c (GSHO 1284) from Parkland (CIho 10001) via R.I. Wolfe's Multiple Marker Stock for Chromosome 5 in Bowman (PI 483237)*7 (GSHO 2052, BW628, NGB 22194); nec1.i in Bowman*2 (BW627, NGB 22193).
References:
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Fedak, G., T. Tsuchiya, and S.B. Helgason. 1972. Use of monotelotrisomics for linkage mapping in barley. Can. J. Genet. Cytol. 14:949-957.
3. Franckowiak, J.D. (Unpublished).
4. Jensen, J. 1971. Mapping of 10 mutant genes for necrotic spotting in barley by means of translocation. p. 213-219. In R.A. Nilan (ed.) Barley Genetics II. Proc. Second Int. Barley Genet. Symp., Pullman, WA, 1969. Washington State Univ. Press, Pullman.
5. Jensen, J. 1992. Coordinator's report: Chromosome 5. Barley Genet. Newsl. 21:89-92.
6. Jensen, J., and J.H. Jørgensen. 1973. Locating some genes on barley chromosome 5. Barley Genet. Newsl. 3:25-27.
7. Keiša, A., K. Kanberga-Silina, I. Nakurte, L. Kunga, and N. Rostoks. 2011. Differential disease resistance response in the barley necrotic mutant nec1. BMC Plant Biol. 11:66.
8. Nielsen, G., H. Johansen, and J. Jensen. 1983. Localization on barley chromosome 5 of the locus Pgd2 coding for phosphogluconate dehydrogenase. Barley Genet. Newsl. 13:57-59.
9. Ramage, T., and J.L.A. Eckhoff. 1985. Assignment of mutants in Morex to chromosomes. Barley Genet. Newsl. 15:22-25.
10. Rostoks, N., D. Schmierer, S. Mudie, T. Drader, R. Brueggeman, D. Caldwell, R. Waugh, and A. Kleinhofs. 2006. Barley necrotic locus nec1 encodes the cyclic nucleotide-gated ion channel 4 homologous to the Arabidopsis Hlm1. Mol. Gen. Genomics 275:159-168.
2. Fedak, G., T. Tsuchiya, and S.B. Helgason. 1972. Use of monotelotrisomics for linkage mapping in barley. Can. J. Genet. Cytol. 14:949-957.
3. Franckowiak, J.D. (Unpublished).
4. Jensen, J. 1971. Mapping of 10 mutant genes for necrotic spotting in barley by means of translocation. p. 213-219. In R.A. Nilan (ed.) Barley Genetics II. Proc. Second Int. Barley Genet. Symp., Pullman, WA, 1969. Washington State Univ. Press, Pullman.
5. Jensen, J. 1992. Coordinator's report: Chromosome 5. Barley Genet. Newsl. 21:89-92.
6. Jensen, J., and J.H. Jørgensen. 1973. Locating some genes on barley chromosome 5. Barley Genet. Newsl. 3:25-27.
7. Keiša, A., K. Kanberga-Silina, I. Nakurte, L. Kunga, and N. Rostoks. 2011. Differential disease resistance response in the barley necrotic mutant nec1. BMC Plant Biol. 11:66.
8. Nielsen, G., H. Johansen, and J. Jensen. 1983. Localization on barley chromosome 5 of the locus Pgd2 coding for phosphogluconate dehydrogenase. Barley Genet. Newsl. 13:57-59.
9. Ramage, T., and J.L.A. Eckhoff. 1985. Assignment of mutants in Morex to chromosomes. Barley Genet. Newsl. 15:22-25.
10. Rostoks, N., D. Schmierer, S. Mudie, T. Drader, R. Brueggeman, D. Caldwell, R. Waugh, and A. Kleinhofs. 2006. Barley necrotic locus nec1 encodes the cyclic nucleotide-gated ion channel 4 homologous to the Arabidopsis Hlm1. Mol. Gen. Genomics 275:159-168.
Prepared:
J. Jensen. 1981. Barley Genet. Newsl. 11:101.
Revised:
J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:220.
J.D. Franckowiak. 2007. Barley Genet. Newsl. 37:251-252.
A. Kleinhofs and J.D. Franckowiak. 2013. Barley Genet. Newsl. 43:108-109.
J.D. Franckowiak. 2007. Barley Genet. Newsl. 37:251-252.
A. Kleinhofs and J.D. Franckowiak. 2013. Barley Genet. Newsl. 43:108-109.
