International Database for Barley Genes and Barley Genetic Stocks
BGS 526, Eceriferum-ym, cer-ym
Stock number: BGS 526
Locus name: Eceriferum-ym
Locus symbol: cer-ym
Previous nomenclature and gene symbolization:
None.
Inheritance:
Monofactorial recessive (7).
Located in chromosome 4HL (1, 3, 4); cer-ym.753 is associated with SNP markers 2_0289 to 1_1513 (positions 69.62 to 101.44 cM) in 4H bins 05 to 07 of the Bowman backcross-derived line BW144 (1); cer-zv.268, a possible allele (4), is associated with SNP markers 2_1122 to 1_0568 (positions 47.80 to 78.18 cM) in 4H bins 05 to 06 of the Bowman backcross-derived line BW177 (1); cer-ym.753 and cer-zv.268 are in a 0.8 cM interval between EST markers AK370363 and AK251484 in pericentromeric region of 4H (3, 4)..
Located in chromosome 4HL (1, 3, 4); cer-ym.753 is associated with SNP markers 2_0289 to 1_1513 (positions 69.62 to 101.44 cM) in 4H bins 05 to 07 of the Bowman backcross-derived line BW144 (1); cer-zv.268, a possible allele (4), is associated with SNP markers 2_1122 to 1_0568 (positions 47.80 to 78.18 cM) in 4H bins 05 to 06 of the Bowman backcross-derived line BW177 (1); cer-ym.753 and cer-zv.268 are in a 0.8 cM interval between EST markers AK370363 and AK251484 in pericentromeric region of 4H (3, 4)..
Description:
Surface wax coating on the spike, the leaf sheath and stem, and the leaf blade appears absent (wax code - - -) (7). Plants are weak, culms have a kinked peduncle, nodes are wax covered, and kernels are small, globe shaped, and nearly hull-less (2, 5). Plants of the Bowman backcross-derived line for cer-ym.753, BW144, averaged about 2/3 the height of Bowman with peduncles and leaf blades about 2/3 normal length. Awns of BW144 were slightly shorter and kernels were smaller and lighter, 4.2 vs. 6.1 mg. The grain yields of BW144 were less than 1/3 that of Bowman. Plants of the Bowman backcross-derived line for cer-zv.268 (BW177) were phenotypically similar to BW144 (cer-ym.753) plants, but they were weaker (2, 3). Detached leaves of the mutant cer-ym.753 lost 41.8% of their initial weight after 1 hour of dehydration under laboratory conditions, while that of the wild type Bowman plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining (3, 4). The cer-ym.753 leaf blades showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency (3, 4). Allelism tests demonstrated that mutants at the cer-ym and cer-zv loci are not alleles (5).
 leaf sheath to the left compared with normal Bonus'))
 leaf sheath to the left compared with normal Bowman'))
 leaf blade seedlings with waterdrops in Bowman'))
 spike showing elongated globe shaped grains to the left compared with normal Bowman'))
 culm showing wax covered node to the right compered with normal Bowman.'))
Origin of mutant:
An ethylene oxide induced mutant in Bonus (NGB 14657, PI 189763) (5).
Mutational events:
cer-ym.130, (NGB 111015), -ym.753 (NGB 111641, GSHO 1546) in Bonus (NGB 14657, PI 189763) (7); cer-zv.268 (NGB 111155, GSHO 1529), -zv.342 (NGB 111229), -zv.576 (NGB 111464) in Foma (NGB 14659, CIho 11333) (6); cer-zv.1256 (NGB 112144) in Kristina (NGB 14661, NGB 1500) (8).
Mutant used for description and seed stocks:
cer-ym.753 (NGB 111641, GSHO 1546) in Bonus; cer-ym.753 in Bowman (PI 483237)*5 (GSHO 2217); cer-ym.753 in Bowman*7 (BW144, NGB 20550).
References:
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendrarnin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627
2. Franckowiak, J.D. (Unpublished).
3. Li, C., C. Liu, X. Ma, A. Wang, R. Duan, C. Nawrath, T. Komatsuda, and G. Chen. 2015. Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity. Breed. Sci. 65:327-332.
4. Li, C., A. Wang, X. Ma, M. Pourkheirandish, S. Sakuma, N. Wang, S. Ning, E. Nevo, C. Nawrath, T. Komatsuda, and G. Chen. 2013. An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves. Theor. Appl. Genet. 126:637-646.
5. Lundqvist, U. (Unpublished).
6. Lundqvist, U., and D. von Wettstein. 1973. Stock list for the eceriferum mutants II. Barley Genet. Newsl. 3:110-112.
7. Lundqvist, U., and D. von Wettstein. 1977. Stock list for the eceriferum mutants IV. Barley Genet. Newsl. 7:92-96.
8. Lundqvist, U., and D. von Wettstein. 1979. Stock list for the eceriferum mutants V. Barley Genet. Newsl. 9:135-137.
2. Franckowiak, J.D. (Unpublished).
3. Li, C., C. Liu, X. Ma, A. Wang, R. Duan, C. Nawrath, T. Komatsuda, and G. Chen. 2015. Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity. Breed. Sci. 65:327-332.
4. Li, C., A. Wang, X. Ma, M. Pourkheirandish, S. Sakuma, N. Wang, S. Ning, E. Nevo, C. Nawrath, T. Komatsuda, and G. Chen. 2013. An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves. Theor. Appl. Genet. 126:637-646.
5. Lundqvist, U. (Unpublished).
6. Lundqvist, U., and D. von Wettstein. 1973. Stock list for the eceriferum mutants II. Barley Genet. Newsl. 3:110-112.
7. Lundqvist, U., and D. von Wettstein. 1977. Stock list for the eceriferum mutants IV. Barley Genet. Newsl. 7:92-96.
8. Lundqvist, U., and D. von Wettstein. 1979. Stock list for the eceriferum mutants V. Barley Genet. Newsl. 9:135-137.
NGB number url references to Nordic Baltic Genebankes Information System (GENBIS):
- NGB 111015 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 111015)
- NGB 111641 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 111641)
Prepared:
U. Lundqvist and J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:453.
Revised:
U. Lundqvist and J.D. Franckowiak. 2016. Barley Genet. Newsl. 46:118-119.
U. Lundqvist and J.D. Franckowiak. 2017. Barley Genet. Newsl. 47:173-174.
U. Lundqvist and J.D. Franckowiak. 2017. Barley Genet. Newsl. 47:173-174.
