BGS 326, Broad leaf 1, blf1

Bredbladig = bb (7).
Latifolium (4).
Broad leaf = bb (6).
Broad leaf 1-1 = blf1-1 (3).

Monofactorial recessive (4, 7).
Located in chromosome 5HL (7); blf1.a is about 20.6 cM proximal from the srh1 (short rachilla hair 1) locus (7); blf1.a (blf1-1) is positioned in 5HL at 65 cM (3). The blf1.a mutant is associated with SNP markers 1_0525 to 2_1078 (positions 65.03 to 118.78 cM) in 2H bins 07 to 08 of the Bowman backcross-derived line BW058 (SNP associations on 3HL and 6HL are shorter) (1).

Plants are somewhat lighter green than normal. All leaf blades are very broad, about twice normal width, and markedly crinkled, especially at the margins (4). Leaf blades of flag minus 1 leaves of the Bowman backcross-derived line for blf1.a, BW058 are nearly twice as wide as those of Bowman. Heading of BW058 plants was delayed by 5 to 10 days compared to Bowman; plants were slightly shorter; and kernel size and grain yield are slightly less (2). The comparison of the blf1.a (blf1-1) mutant plants with its parent, Bonus, demonstrated an increase cell number across the leaf blades and greater number of vascular bundles and stomata. Also, the width of the lemma, palea, and kernel were increased in the mutant (3). The BLF1 gene encodes a presumed transcriptional regulator of the INDETERMINATE DOMAIN (IDD) family and shows a dynamic expression pattern in the shoot apical meristem and young leaf primordia (3). The normal BLF1 gene regulates barley leaf size by restricting cell proliferation in the leaf-width direction (3).

An X-ray induced mutant in Bonus (NGB 14657, PI 189763) (4, 7).

blf1.a (Alb. Acc. 55, GSHO 1393) in Bonus (NGB 14657, PI 189763) (7), identified as blf1-1 by Jöst et al. (3); blf1-2, blf1-3, blf1-4, blf1-5 named as mutants in the blf1 locus isolated from the Barke TILLING Population (3); blf1.7 (blf1-6) (NGB 117240), blf1.8 (blf1-6) (NGB 117241) in Bonus (3, 5); blf1.3 (NGB 117237), blf1.5 (NGB 117238) in Bonus having a complete deletion of the blf1 locus (3, 5).

blf1.a (GSHO 1393) in Bonus; blf1.a in Bowman (PI 483237)*1 (GSHO 2166), blf1.a in Bowman*3 (BW058, NGB 20466).

1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendramin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Gustafsson, Å., A. Hagberg, U. Lundqvist, and G. Persson. 1969. A proposed system of symbols for the collection of barley mutants at Svalöv. Hereditas 62:409-414.
4. Jöst, M., G. Hensel, C. Kappel, A. Druka, A. Sicard, U. Hohmann, S. Beier, A. Himmelbach, R. Waugh, J. Kumlehn, N. Stein, and M. Lenhard. 2016. The INDETERMINATE DOMAIN protein BROAD LEAF1 limits barley leaf width by restricting lateral proliferation. Curr. Biol. 26:903-909.
5. Lundqvist, U. (Unpublished).
6. Robertson, D.W., G.A. Wiebe, R.G. Shands, and A. Hagberg. 1965. A summary of linkage studies in cultivated barley, Hordeum species: Supplement III, 1954-1963. Crop Sci. 5:33-43.
7. Walker, G.W.R., J. Dietrich, R. Miller, and K.J. Kasha. 1963. Recent barley mutants and their linkages II. Genetic data for further mutants. Can. J. Genet. Cytol. 5:200-219.

U. Lundqvist and J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:274.

J.D. Franckowiak and U. Lundqvist. 2011. Barley Genet. Newsl. 41:130.
U. Lundqvist and J.D. Franckowiak. 2016. Barley Genet. Newsl. 46:90-91.