International Database for Barley Genes and Barley Genetic Stocks
BGS 439, Eceriferum-zv, cer-zv
Stock number: BGS 439
Locus name: Eceriferum-zv
Locus symbol: cer-zv
Previous nomenclature and gene symbolization:
None.
Inheritance:
Monofactorial recessive (6).
Located in chromosome 4HL (1, 3, 4); cer-zv.268 is associated with SNP markers 2_1122 to 1_0568 (positions 47.80 to 78.18 cM) in 4H bins 05 to 06 of the Bowman backcross-derived line BW177 (1); cer-ym.753, a possible allele (4), is associated with SNP markers 2_0289 to 1_1513 (positions 69.62 to 101.44 cM) in 4H bins 05 to 07 of the Bowman backcross-derived line BW144 (1); cer-zv.268 and cer-ym.753 are in a 0.8 cM interval between EST markers AK370363 and AK251484 in pericentromeric region of 4H (3, 4).
Located in chromosome 4HL (1, 3, 4); cer-zv.268 is associated with SNP markers 2_1122 to 1_0568 (positions 47.80 to 78.18 cM) in 4H bins 05 to 06 of the Bowman backcross-derived line BW177 (1); cer-ym.753, a possible allele (4), is associated with SNP markers 2_0289 to 1_1513 (positions 69.62 to 101.44 cM) in 4H bins 05 to 07 of the Bowman backcross-derived line BW144 (1); cer-zv.268 and cer-ym.753 are in a 0.8 cM interval between EST markers AK370363 and AK251484 in pericentromeric region of 4H (3, 4).
Description:
Surface wax coating on the spike, the leaf sheath and stem, and the leaf blade appears absent (wax code - - -) (6). Plants are semidwarf, very weak, and head late, and the hull is poorly attached to the kernel. The Bowman backcross-derived line for cer-zv.268, BW177, is a semidwarf (2/3 normal height) with a kinked upper peduncle and a strong wax coating on the nodes (2). Compared to Bowman, BW177 plants headed 2 to 10 days later in various environments. Plant height, peduncle length, and leaf blade length measurements were 1/2 to 2/3 those for Bowman. Awns were slightly shorter, but rachis internodes were slightly longer. Kernels of BW177 were shorter and thinner and weighed less, 3.5 vs. 5.8 mg, compared to Bowman kernels. Grain yields of BW177 averaged 10% those of Bowman (2). The cer-ym.753 plants are weak, culms have a kinked peduncle, nodes are wax covered, and kernels are small, globe shaped, and nearly hulless (2, 5). Plants of the Bowman backcross-derived line for cer-ym.753, BW144, were phenotypically similar to BW177 plants, but they were more vigorous (2, 3, 4). Detached leaves of the mutant cer-ym.753 lost 41.8% of their initial weight after 1 hour of dehydration under laboratory conditions, while that of the wild type Bowman plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The cer-ym.753 leaf blades showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency (3, 4). Allelism tests demonstrated that mutants at the cer-zv and cer-ym loci are not alleles (5).
 leaf blades and sheath to the left compared with normal Foma'))
 spike in Foma'))
 seedling leaves with waterdrops on the mutants in Bowman'))
 in Bowman'))
 globe shaped grains with curled awns to the left compared with a single normal Bowman grain'))
 plant showing absent wax coating on spike, leaf sheath and leaf blade to the left compared with normal Bowman'))
 plant showing absent surface wax coating on spike, leaf sheath, leaf blade and wax covered nodes at heading time to the left compared with normal Bowman'))
 culm showing the wax covered node to the right compared with normal Bowman.'))
Origin of mutant:
An ethylene imine induced mutant in Foma (NGB 14659, CIho 11333) (5).
Mutational events:
cer-zv.268 (NGB 111155, GSHO 1529), -zv.342 (NGB 111229), -zv.576 (NGB 111464) in Foma (NGB 14659, CIho 11333) (6); cer-zv.1256 (NGB 112144) in Kristina (NGB 14661, NGB 1500) (8). cer-ym.130, (NGB 111015, GSHO 1546), -ym.753 (NGB 111641) in Bonus (NGB 14657, PI 189763) (7).
Mutant used for description and seed stocks:
cer-zv.268 (NGB 111155, GSHO 1529) in Foma; cer-zv.268 in Bowman (PI 483237)*5 (GSHO 2207); cer-zv.268 in Bowman *7 (BW177, NGB 22009); cer-ym.753 (NGB 111641, GSHO 1546) in Bonus; cer-ym.753 in Bowman (PI 483237)*5 (GSHO 2217); cer-ym.753 in Bowman*7 (BW144, NGB 20550).
References:
1. Druka, A., J. Franckowiak, U. Lundqvist, N. Bonar, J. Alexander, K. Houston, S. Radovic, F. Shahinnia, V. Vendrarnin, M. Morgante, N. Stein, and R. Waugh. 2011. Genetic dissection of barley morphology and development. Plant Physiol. 155:617-627.
2. Franckowiak, J.D. (Unpublished).
3. Li, C., C. Liu, X. Ma, A. Wang, R. Duan, C. Nawrath, T. Komatsuda, and G. Chen. 2015. Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity. Breed. Sci. 65:327-332.
4. Li, C., A. Wang, X. Ma, M. Pourkheirandish, S. Sakuma, N. Wang, S. Ning, E. Nevo, C. Nawrath, T. Komatsuda, and G. Chen. 2013. An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves. Theor. Appl. Genet. 126:637-646.
5. Lundqvist, U. (Unpublished).
6 Lundqvist, U., and D. von Wettstein. 1973. Stock list for the eceriferum mutants II. Barley Genet. Newsl. 3:110-112.
7. Lundqvist, U., and D. von Wettstein. 1977. Stock list for the eceriferum mutants IV. Barley Genet. Newsl. 7:92-96.
8. Lundqvist, U., and D. von Wettstein. 1979. Stock list for the eceriferum mutants V. Barley Genet. Newsl. 9:135-137.
2. Franckowiak, J.D. (Unpublished).
3. Li, C., C. Liu, X. Ma, A. Wang, R. Duan, C. Nawrath, T. Komatsuda, and G. Chen. 2015. Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity. Breed. Sci. 65:327-332.
4. Li, C., A. Wang, X. Ma, M. Pourkheirandish, S. Sakuma, N. Wang, S. Ning, E. Nevo, C. Nawrath, T. Komatsuda, and G. Chen. 2013. An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves. Theor. Appl. Genet. 126:637-646.
5. Lundqvist, U. (Unpublished).
6 Lundqvist, U., and D. von Wettstein. 1973. Stock list for the eceriferum mutants II. Barley Genet. Newsl. 3:110-112.
7. Lundqvist, U., and D. von Wettstein. 1977. Stock list for the eceriferum mutants IV. Barley Genet. Newsl. 7:92-96.
8. Lundqvist, U., and D. von Wettstein. 1979. Stock list for the eceriferum mutants V. Barley Genet. Newsl. 9:135-137.
NGB number url references to Nordic Baltic Genebankes Information System (GENBIS):
- NGB 111155 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 111155)
- NGB 111229 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 111229)
- NGB 111464 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 111464)
- NGB 112144 (https://www.nordic-baltic-genebanks.org/gringlobal/accessiondetail?accid=NGB 112144)
Prepared:
U. Lundqvist. 1975. Barley Genet. Newsl. 5:157
Revised:
U. Lundqvist and J.D. Franckowiak. 1997. Barley Genet. Newsl. 26:391.
U. Lundqvist and J.D. Franckowiak. 2016. Barley Genet. Newsl. 46:109-110.
U. Lundqvist and J.D. Franckowiak. 2017. Barley Genet. Newsl. 47:133-134.
U. Lundqvist and J.D. Franckowiak. 2016. Barley Genet. Newsl. 46:109-110.
U. Lundqvist and J.D. Franckowiak. 2017. Barley Genet. Newsl. 47:133-134.
